Acknowledgments

This work is supported by the Natural Science Foundation of China (31270831 and 30970630), the Outstanding Youth Science Foundation of Chongqing (cstc2011jjjq10003), the Program for New Century Excellent Talents in University (NCET-08-0912), the Fundamental Research Funds for the Central Universities (XDJK2016C158) and the Doctoral Fund of Southwest University (XJKJXM003338).

Nhs Activation Of Sepharose 2b 1

Data File 28-9067-88 AB Protein Sample Preparation NHS HP SpinTrap NHS HP SpinTrap Buffer Kit. NHS-activated Sepharose High Performance is provided. (Fig 2A and 2B). Recovery of the start material was 80% to 95% with the majority of the protein.

Supporting information

• Thermo Scientific Pierce NHS-Activated Agarose is a high-quality, amine-reactive, beaded-agarose resin for rapid and stable immobilization of proteins, peptides and other ligands via primary amines.
• RESULTS: We immobilized Ulp1 on N-hydroxysuccinimide (NHS)-activated Sepharose with a coupling efficiency of 1.7 mg/ml. The immobilized Ulp1 maintains 95% substrate-cleavage ability and significantly enhances pH and thermal stability, especially can withstand pH of 10.5.

Nhs Activation Of Sepharose 2b

Supplementary Fig. 1—Different immobilization strategies for Ulp1.

Supplementary Fig. 2—DMSO inhibits the degradation of SUMO.

Supplementary Fig. 3—The influence of small molecules on the activity of Ulp1.

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Supplementary Fig. 4—Storage stability of free and immobilized Ulp1 at 30 °C.